Hotspot

trnS-GCU-trnG-UCC

LSC · 8977-9837 · 861 bp

Back to Xylia

Pi 0.03
MarkerSeek score 74.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
861
variable_sites
25
pi
0.03
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 973-1279 bp 1.0 78.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 972-1278 bp 1.0 78.0
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1027-1333 bp 1.0 76.6
trnS-GCU-trnG-UCC_p4 TGGAAACGGAAAGAGAGGGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.3 57.3 1032-1338 bp 1.0 75.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 941-1247 bp 1.0 75.6