Hotspot

ycf1

SSC · 124238-129919 · 5682 bp

Back to Xerophyta

Pi 0.0171

MarkerSeek score 56.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 334
pi: 0.0171
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGAGGGGAGTGCACATTTG	CCCTTACCCTTGTTCGGAAG	50.0	55.0	58.1	57.9	1414-1495 bp	0.928571	83.6
ycf1_p2	GGTTGGCTATCAATCCCGTT	CCCTTACCCTTGTTCGGAAG	50.0	55.0	57.9	57.9	876 bp	0.142857	44.4
ycf1_p3	GGTTGGCTATCAATCCCGTT	AATGGACCATATCGCGGAAC	50.0	50.0	57.9	58.1	212 bp	0.142857	44.3
ycf1_p4	GGTTGGCTATCAATCCCGTT	TTGAATGGACCATATCGCGG	50.0	50.0	57.9	58.1	215 bp	0.142857	44.3
ycf1_p5	GGTTGGCTATCAATCCCGTT	AAGAGGGATCCGACGAAGAA	50.0	50.0	57.9	58.2	898 bp	0.142857	44.1