Hotspot

ndhF

SSC · 110893-113130 · 2238 bp

Back to Xerophyta

Pi 0.0219

MarkerSeek score 64.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 152
pi: 0.0219
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATGGACCATATCGCGGAAC	TGGGTAATCACCTCTGCTTTG	50.0	47.6	58.1	57.9	2483-2533 bp	0.714286	64.8
ndhF_p2	AATGGACCATATCGCGGAAC	GGGTAATCACCTCTGCTTTGT	50.0	47.6	58.1	57.9	2482-2532 bp	0.714286	64.8
ndhF_p3	TTGAATGGACCATATCGCGG	TGGGTAATCACCTCTGCTTTG	50.0	47.6	58.1	57.9	2486-2536 bp	0.714286	64.7
ndhF_p4	TTGAATGGACCATATCGCGG	GGGTAATCACCTCTGCTTTGT	50.0	47.6	58.1	57.9	2485-2535 bp	0.714286	64.7
ndhF_p5	GAATGGACCATATCGCGGAA	TGGGTAATCACCTCTGCTTTG	50.0	47.6	57.8	57.9	2484-2534 bp	0.714286	64.4