Hotspot

ndhG-ndhI

SSC · 119097-119457 · 361 bp

Back to Xerophyta

Pi 0.0391

MarkerSeek score 73.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 45
pi: 0.0391
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TTTCATGTATTGGCCCAGGT	TGATTCAAGAACGATTACCA	45.0	35.0	57.1	52.2	297-438 bp	1.0	52.0
ndhG-ndhI_p2	TTTCATGTATTGGCCCAGGT	TGATTCAAGAACGATTACCAA	45.0	33.3	57.1	53.2	297-438 bp	1.0	52.0
ndhG-ndhI_p3	ATTGGCCCAGGTAAATCCAT	TGATTCAAGAACGATTACCA	45.0	35.0	56.8	52.2	289-430 bp	1.0	52.0
ndhG-ndhI_p4	ATTGGCCCAGGTAAATCCAT	TGATTCAAGAACGATTACCAA	45.0	33.3	56.8	53.2	289-430 bp	1.0	52.0
ndhG-ndhI_p5	GTATTGGCCCAGGTAAATCCA	TGATTCAAGAACGATTACCA	47.6	35.0	57.7	52.2	291-432 bp	1.0	52.0