Hotspot

trnL-UAG-ccsA

SSC · 114667-114749 · 83 bp

Back to Xerophyta

Pi 0.0723

MarkerSeek score 69.60

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 83
variable_sites: 16
pi: 0.0723
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGGATCCGCATAAGAGAGCT	50.0	50.0	57.9	58.0	596-607 bp	1.0	84.8
trnL-UAG-ccsA_p2	TAAGCCGCCATGGTGAAATT	AGGATCCGCATAAGAGAGCT	45.0	50.0	58.2	58.0	618-629 bp	1.0	84.3
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	AGGATCCGCATAAGAGAGCT	45.0	50.0	57.8	58.0	603-614 bp	1.0	84.2
trnL-UAG-ccsA_p4	TAGACACGCTGCTCTTAGGA	GGATCCGCATAAGAGAGCTG	50.0	55.0	57.9	57.9	595-606 bp	1.0	84.2
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	AGGATCCGCATAAGAGAGCT	55.0	50.0	57.8	58.0	597-608 bp	1.0	84.2