Hotspot

rpl22-rps19

LSC · 83409-83533 · 125 bp

Back to Xerophyta

Pi 0.052
MarkerSeek score 68.30
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
125
variable_sites
18
pi
0.052
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TACTCTTCGCGCCTTGAAAA AGCGATAATAAATCTCGTCGT 45.0 38.1 57.8 55.1 174-185 bp 1.0 56.2
rpl22-rps19_p2 TTACTCTTCGCGCCTTGAAA AGCGATAATAAATCTCGTCGT 45.0 38.1 57.8 55.1 175-186 bp 1.0 56.2
rpl22-rps19_p3 AATTACTCTTCGCGCCTTGA AGCGATAATAAATCTCGTCGT 45.0 38.1 57.6 55.1 177-188 bp 1.0 54.4
rpl22-rps19_p4 ATTACTCTTCGCGCCTTGAA AGCGATAATAAATCTCGTCGT 45.0 38.1 57.6 55.1 176-187 bp 1.0 54.4
rpl22-rps19_p5 TCAATTACTCTTCGCGCCTT AGCGATAATAAATCTCGTCGT 45.0 38.1 57.6 55.1 179-190 bp 1.0 54.3