Hotspot

psbE-petL

LSC · 64248-65557 · 1310 bp

Back to Xerophyta

Pi 0.0286

MarkerSeek score 73.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1310
variable_sites: 114
pi: 0.0286
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1029-1447 bp	1.0	81.7
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1027-1445 bp	1.0	81.7
psbE-petL_p3	CAGCAAAAGAACGTTCTCCC	AAGCAGCCAGTAGAAAACCG	50.0	50.0	57.3	58.5	1032-1450 bp	1.0	81.5
psbE-petL_p4	CAGCAAAAGAACGTTCTCCC	GCAGCCAGTAGAAAACCGAA	50.0	50.0	57.3	58.5	1030-1448 bp	1.0	81.5
psbE-petL_p5	AGCAAAAGAACGTTCTCCCG	AAGCAGCCAGTAGAAAACCG	50.0	50.0	58.8	58.5	1031-1449 bp	1.0	80.9