Hotspot

psaA-ycf3

LSC · 41041-41762 · 722 bp

Back to Xerophyta

Pi 0.0135

MarkerSeek score 72.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 35
pi: 0.0135
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	CACACCTATTCCAACCGAGG	55.0	55.0	58.0	58.0	933-966 bp	1.0	86.8
psaA-ycf3_p2	GGGGCCCTTAGCTATTGTTC	GAACAGGCCATTCTACAGGG	55.0	55.0	58.0	58.0	912-945 bp	1.0	86.6
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	ACACCTATTCCAACCGAGGA	55.0	50.0	58.0	58.1	932-965 bp	1.0	86.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	CACACCTATTCCAACCGAGG	50.0	55.0	57.9	58.0	892-925 bp	1.0	86.3
psaA-ycf3_p5	GGGGCCCTTAGCTATTGTTC	AGGCCATTCTACAGGGTGAT	55.0	50.0	58.0	58.1	908-941 bp	1.0	86.2