Hotspot

petN-psbM

LSC · 28244-28827 · 584 bp

Back to Xerophyta

Pi 0.0258

MarkerSeek score 68.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 49
pi: 0.0258
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	CGTAAAAACAGTTAGCCAAAACG	55.0	39.1	58.1	57.5	629-656 bp	1.0	60.9
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	CGTAAAAACAGTTAGCCAAAACG	55.0	39.1	58.1	57.5	628-655 bp	1.0	60.9
petN-psbM_p3	TATGGGGAAGGAGTGGACTC	CGTAAAAACAGTTAGCCAAAACG	55.0	39.1	57.8	57.5	627-654 bp	1.0	60.5
petN-psbM_p4	GGGGAAGGAGTGGACTCTAG	CGTAAAAACAGTTAGCCAAAACG	60.0	39.1	58.2	57.5	624-651 bp	1.0	60.2
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	AAAACAGTTAGCCAAAACGA	55.0	35.0	58.1	54.0	624-651 bp	1.0	56.8