Hotspot

rpoB-trnC-GCA

LSC · 26375-27662 · 1288 bp

Back to Xerophyta

Pi 0.0258

MarkerSeek score 72.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 105
pi: 0.0258
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GGTATTGTGGACATTCCCCC	TTGTTTATCAGGCGACACCC	55.0	50.0	57.9	58.2	1338-1463 bp	1.0	87.9
rpoB-trnC-GCA_p2	TGTGGACATTCCCCCATTTC	TTGTTTATCAGGCGACACCC	50.0	50.0	57.8	58.2	1333-1458 bp	1.0	86.7
rpoB-trnC-GCA_p3	CATTCCCCCATTTCCATCCC	TTGTTTATCAGGCGACACCC	55.0	50.0	58.3	58.2	1327-1452 bp	1.0	86.3
rpoB-trnC-GCA_p4	GGTATTGTGGACATTCCCCC	GCGACACCCAGATTTGAACT	55.0	50.0	57.9	58.5	1327-1452 bp	1.0	85.6
rpoB-trnC-GCA_p5	CCATTTCCATCCCAGAGCAT	TTGTTTATCAGGCGACACCC	50.0	50.0	57.6	58.2	1320-1445 bp	1.0	85.5