Hotspot

atpH-atpI

LSC · 13442-14091 · 650 bp

Back to Xerophyta

Pi 0.0426

MarkerSeek score 77.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 93
pi: 0.0426
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTCTACAGCTTGGCCT	CTGCGCCCACGAATGATATA	50.0	50.0	58.0	57.9	1115-1147 bp	1.0	90.2
atpH-atpI_p2	CTCAAATCAGTCCTTCCCGG	CTGCGCCCACGAATGATATA	55.0	50.0	58.0	57.9	1401-1433 bp	1.0	89.4
atpH-atpI_p3	CTCAAATCAGTCCTTCCCGG	GTGTAGAAGTCGGCCAACAT	55.0	50.0	58.0	57.9	1731-1763 bp	1.0	88.3
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CTGCGCCCACGAATGATATA	45.0	50.0	58.0	57.9	1032-1064 bp	0.785714	81.4
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	GTGTAGAAGTCGGCCAACAT	45.0	50.0	58.0	57.9	1362-1394 bp	0.785714	79.9