Hotspot

rps16-trnQ-UUG

LSC · 5519-6473 · 955 bp

Back to Xerophyta

Pi 0.0252
MarkerSeek score 69.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
955
variable_sites
81
pi
0.0252
unique_haplotype_count
14
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGGCGATCATGTCCTTCAA CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1559-1610 bp 0.928571 85.7
rps16-trnQ-UUG_p2 GGCGATCATGTCCTTCAAGT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1557-1608 bp 0.928571 85.7
rps16-trnQ-UUG_p3 CAGGCGATCATGTCCTTCAA GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1048-1104 bp 0.714286 77.3
rps16-trnQ-UUG_p4 GGCGATCATGTCCTTCAAGT GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1046-1102 bp 0.714286 77.3
rps16-trnQ-UUG_p5 AATCCACAGGCGATCATGTC GAAATCAAAATGGGGCGTGG 50.0 50.0 58.0 58.0 1054-1110 bp 0.714286 77.2