Hotspot

petD

LSC · 75756-76934 · 1179 bp

Back to Xeranthemum

Pi 0.00509

MarkerSeek score 62.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1179
variable_sites: 9
pi: 0.00509
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1313-1319 bp	1.0	77.6
petD_p2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1312-1318 bp	1.0	77.6
petD_p3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1316-1322 bp	1.0	77.3
petD_p4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1315-1321 bp	1.0	77.3
petD_p5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	57.7	58.4	1316-1322 bp	1.0	75.0