Hotspot

trnW-CCA-trnP-UGG

LSC · 65747-65910 · 164 bp

Back to Xeranthemum

Pi 0.0285
MarkerSeek score 64.40
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
164
variable_sites
7
pi
0.0285
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 243-244 bp 1.0 81.5
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 244-245 bp 1.0 81.5
trnW-CCA-trnP-UGG_p3 AGAAGATACGAATCGCGCTC GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 289-290 bp 1.0 81.1
trnW-CCA-trnP-UGG_p4 GAAGATACGAATCGCGCTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 288-289 bp 1.0 81.1
trnW-CCA-trnP-UGG_p5 GAAGATACGAATCGCGCTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 289-290 bp 1.0 81.1