Hotspot

petA-psbJ

LSC · 62418-63200 · 783 bp

Back to Xeranthemum

Pi 0.00943

MarkerSeek score 66.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 11
pi: 0.00943
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	903-921 bp	1.0	79.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	893-911 bp	1.0	79.7
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	941-959 bp	1.0	77.9
petA-psbJ_p4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	940-958 bp	1.0	77.9
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	931-949 bp	1.0	77.9