Hotspot

ndhC-trnV-UAC

LSC · 49538-50728 · 1191 bp

Back to Xeranthemum

Pi 0.0124
MarkerSeek score 56.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1191
variable_sites
22
pi
0.0124
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GAGCTAAAACTCCCGAAATGA AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.0 57.8 1304-1321 bp 1.0 57.4
ndhC-trnV-UAC_p2 GAGCTAAAACTCCCGAAATGAA AGAAGGTCTACGGTTCGAGT 40.9 50.0 56.7 57.8 1304-1321 bp 1.0 55.2
ndhC-trnV-UAC_p3 AGCTAAAACTCCCGAAATGA AGAAGGTCTACGGTTCGAGT 40.0 50.0 54.7 57.8 1303-1320 bp 1.0 54.8
ndhC-trnV-UAC_p4 GAGCTAAAACTCCCGAAATGA GAAGGTCTACGGTTCGAGTC 42.9 55.0 56.0 57.5 1303-1320 bp 1.0 54.6
ndhC-trnV-UAC_p5 AGCTAAAACTCCCGAAATGAA AGAAGGTCTACGGTTCGAGT 38.1 50.0 55.5 57.8 1303-1320 bp 1.0 53.6