Hotspot

trnT-UGU-trnL-UAA

LSC · 45648-46201 · 554 bp

Back to Xeranthemum

Pi 0.0189

MarkerSeek score 70.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 15
pi: 0.0189
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	603-610 bp	1.0	83.4
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.8	57.8	635-642 bp	1.0	82.3
trnT-UGU-trnL-UAA_p3	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	605-612 bp	1.0	80.3
trnT-UGU-trnL-UAA_p4	TGCGATGCTCTAACCTCTGA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.5	57.8	604-611 bp	1.0	80.2
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AAGTCCGTAGCGTCTACCTA	55.0	50.0	57.9	57.3	612-619 bp	1.0	78.9