Hotspot

rps14-psaB

LSC · 36191-36322 · 132 bp

Back to Xeranthemum

Pi 0.0227

MarkerSeek score 63.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 132
variable_sites: 4
pi: 0.0227
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGCCTCTTCTTTTCCCTCTG	CGGTGGCACTTTCCATTGTA	50.0	50.0	57.1	58.5	289-290 bp	1.0	70.4
rps14-psaB_p2	TGCCTCTTCTTTTCCCTCTG	CGGCTTTCTTGATTGCCTCT	50.0	50.0	57.1	58.5	214-215 bp	1.0	70.2
rps14-psaB_p3	TGTCACTCAACGATCGAACT	CGGTGGCACTTTCCATTGTA	45.0	50.0	56.9	58.5	366-367 bp	1.0	68.5
rps14-psaB_p4	TTGCCTCTTCTTTTCCCTCTG	CGGTGGCACTTTCCATTGTA	47.6	50.0	57.9	58.5	290-291 bp	1.0	68.2
rps14-psaB_p5	TGTCACTCAACGATCGAACT	CGGCTTTCTTGATTGCCTCT	45.0	50.0	56.9	58.5	291-292 bp	1.0	68.1