Hotspot

atpF-atpA

LSC · 27613-27677 · 65 bp

Back to Xeranthemum

Pi 0.0219

MarkerSeek score 70.10

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0219
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TGGGGGCGATGAAAGAAAAA	ATTTCGTCGGCTTGAATGGT	45.0	45.0	58.0	58.2	114-120 bp	1.0	83.9
atpF-atpA_p2	TTGGGGGCGATGAAAGAAAA	ATTTCGTCGGCTTGAATGGT	45.0	45.0	58.0	58.2	115-121 bp	1.0	83.9
atpF-atpA_p3	TGGGGGCGATGAAAGAAAAA	GTCGGCTTGAATGGTTACCA	45.0	50.0	58.0	58.2	109-115 bp	1.0	83.9
atpF-atpA_p4	TTGGGGGCGATGAAAGAAAA	GTCGGCTTGAATGGTTACCA	45.0	50.0	58.0	58.2	110-116 bp	1.0	83.9
atpF-atpA_p5	TGGGGGCGATGAAAGAAAAA	TCGTCGGCTTGAATGGTTAC	45.0	50.0	58.0	58.3	111-117 bp	1.0	83.1