Hotspot

petN-psbM

LSC · 9991-10509 · 519 bp

Back to Xeranthemum

Pi 0.0186

MarkerSeek score 58.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 14
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGACTCGCGAGAATCTCTGA	TTCCCGCGACTCATCTTTTT	50.0	45.0	58.0	57.8	1372-1389 bp	1.0	81.3
petN-psbM_p2	TTTCCCCAGTTCAAATCCGG	TTCCCGCGACTCATCTTTTT	50.0	45.0	58.1	57.8	1712-1723 bp	1.0	80.9
petN-psbM_p3	AGACTCGCGAGAATCTCTGA	TCCCGCGACTCATCTTTTTC	50.0	50.0	58.0	58.3	1371-1388 bp	1.0	80.5
petN-psbM_p4	GTATCATTTTGGCGGCATGG	TTCCCGCGACTCATCTTTTT	50.0	45.0	57.9	57.8	1764-1775 bp	1.0	80.4
petN-psbM_p5	AAGCAAGACTTCCCGATTCC	TTCCCGCGACTCATCTTTTT	50.0	45.0	57.9	57.8	1506-1509 bp	0.666667	64.7