Hotspot

trnK-UUU-rps16

LSC · 4314-5137 · 824 bp

Back to Xeranthemum

Pi 0.0174

MarkerSeek score 70.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 20
pi: 0.0174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGTGGTCTTGCAAACTT	TTCCTTGAAAAAGGCGCTCA	45.0	45.0	58.0	58.3	862-1043 bp	1.0	82.8
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	779-960 bp	1.0	81.9
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	778-959 bp	1.0	81.9
trnK-UUU-rps16_p4	ATCAGTCGTGGTCTTGCAAA	TTCCTTGAAAAAGGCGCTCA	45.0	45.0	57.7	58.3	865-1046 bp	1.0	81.1
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	794-975 bp	1.0	81.1