primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GAGCTTAGTTTCCGTCTGGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	78	461	987-1076	1.000000	84.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	78	461	993-1082	1.000000	84.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	GGAGCTTAGTTTCCGTCTGG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	78	461	988-1077	1.000000	84.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GGGAGCTTAGTTTCCGTCTG	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.140460	78	461	989-1078	1.000000	84.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	78	461	1001-1090	1.000000	84.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGTGGTCTTGCAAACTT	TTCCTTGAAAAAGGCGCTCA	20	20	45.0	45.0	58.0	58.3	0.0	7.6	0.352032	4314	5137	862-1043	1.000000	82.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4314	5137	779-960	1.000000	81.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4314	5137	778-959	1.000000	81.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	ATCAGTCGTGGTCTTGCAAA	TTCCTTGAAAAAGGCGCTCA	20	20	45.0	45.0	57.7	58.3	0.0	7.6	0.568191	4314	5137	865-1046	1.000000	81.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4314	5137	794-975	1.000000	81.1
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	1.042943	8326	8472	252-263	1.000000	76.9
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	20	20	50.0	55.0	57.1	57.6	0.0	0.0	1.329655	8326	8472	200-211	1.000000	74.9
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.335793	8326	8472	252-263	1.000000	74.6
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	21	20	52.4	50.0	57.7	58.1	0.0	0.0	1.434740	8326	8472	271-282	1.000000	73.4
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	20	20	50.0	55.0	57.1	58.6	0.0	0.0	1.569752	8326	8472	243-254	1.000000	72.7
petN-psbM_p1	petN-psbM	1	AGACTCGCGAGAATCTCTGA	TTCCCGCGACTCATCTTTTT	20	20	50.0	45.0	58.0	57.8	40.6	8.2	0.210170	9991	10509	1372-1389	1.000000	81.3
petN-psbM_p2	petN-psbM	2	TTTCCCCAGTTCAAATCCGG	TTCCCGCGACTCATCTTTTT	20	20	50.0	45.0	58.1	57.8	0.0	8.2	0.261040	9991	10509	1712-1723	1.000000	80.9
petN-psbM_p3	petN-psbM	3	AGACTCGCGAGAATCTCTGA	TCCCGCGACTCATCTTTTTC	20	20	50.0	50.0	58.0	58.3	40.6	8.2	0.309247	9991	10509	1371-1388	1.000000	80.5
petN-psbM_p4	petN-psbM	4	GTATCATTTTGGCGGCATGG	TTCCCGCGACTCATCTTTTT	20	20	50.0	45.0	57.9	57.8	14.3	8.2	0.317508	9991	10509	1764-1775	1.000000	80.4
petN-psbM_p5	petN-psbM	5	AAGCAAGACTTCCCGATTCC	TTCCCGCGACTCATCTTTTT	20	20	50.0	45.0	57.9	57.8	0.0	8.2	0.294255	9991	10509	1506-1509	0.666667	64.7
atpF-atpA_p1	atpF-atpA	1	TGGGGGCGATGAAAGAAAAA	ATTTCGTCGGCTTGAATGGT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.181289	27613	27677	114-120	1.000000	83.9
atpF-atpA_p2	atpF-atpA	2	TTGGGGGCGATGAAAGAAAA	ATTTCGTCGGCTTGAATGGT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.181289	27613	27677	115-121	1.000000	83.9
atpF-atpA_p3	atpF-atpA	3	TGGGGGCGATGAAAGAAAAA	GTCGGCTTGAATGGTTACCA	20	20	45.0	50.0	58.0	58.2	0.0	6.5	0.186431	27613	27677	109-115	1.000000	83.9
atpF-atpA_p4	atpF-atpA	4	TTGGGGGCGATGAAAGAAAA	GTCGGCTTGAATGGTTACCA	20	20	45.0	50.0	58.0	58.2	0.0	6.5	0.186431	27613	27677	110-116	1.000000	83.9
atpF-atpA_p5	atpF-atpA	5	TGGGGGCGATGAAAGAAAAA	TCGTCGGCTTGAATGGTTAC	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.285312	27613	27677	111-117	1.000000	83.1
rps14-psaB_p1	rps14-psaB	1	TGCCTCTTCTTTTCCCTCTG	CGGTGGCACTTTCCATTGTA	20	20	50.0	50.0	57.1	58.5	0.0	18.5	1.337883	36191	36322	289-290	1.000000	70.4
rps14-psaB_p2	rps14-psaB	2	TGCCTCTTCTTTTCCCTCTG	CGGCTTTCTTGATTGCCTCT	20	20	50.0	50.0	57.1	58.5	0.0	0.0	1.409763	36191	36322	214-215	1.000000	70.2
rps14-psaB_p3	rps14-psaB	3	TGTCACTCAACGATCGAACT	CGGTGGCACTTTCCATTGTA	20	20	45.0	50.0	56.9	58.5	24.3	18.5	1.550598	36191	36322	366-367	1.000000	68.5
rps14-psaB_p4	rps14-psaB	4	TTGCCTCTTCTTTTCCCTCTG	CGGTGGCACTTTCCATTGTA	21	20	47.6	50.0	57.9	58.5	0.0	18.5	1.616879	36191	36322	290-291	1.000000	68.2
rps14-psaB_p5	rps14-psaB	5	TGTCACTCAACGATCGAACT	CGGCTTTCTTGATTGCCTCT	20	20	45.0	50.0	56.9	58.5	24.3	0.0	1.622477	36191	36322	291-292	1.000000	68.1
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	40806	41429	816-816	1.000000	76.7
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	40806	41429	818-818	1.000000	76.7
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	20	20	55.0	45.0	57.8	57.5	18.5	0.0	0.716540	40806	41429	817-817	1.000000	74.9
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	40806	41429	751-751	1.000000	74.7
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	40806	41429	753-753	1.000000	74.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.283137	45648	46201	603-610	1.000000	83.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	57.8	57.8	15.5	0.0	0.390891	45648	46201	635-642	1.000000	82.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	ATGCGATGCTCTAACCTCTG	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.673233	45648	46201	605-612	1.000000	80.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TGCGATGCTCTAACCTCTGA	GCGTCTACCTATTTCGCCAT	20	20	50.0	50.0	58.5	57.8	0.0	0.0	0.676068	45648	46201	604-611	1.000000	80.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AAGTCCGTAGCGTCTACCTA	20	20	55.0	50.0	57.9	57.3	0.0	10.2	0.831595	45648	46201	612-619	1.000000	78.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GAGCTAAAACTCCCGAAATGA	AGAAGGTCTACGGTTCGAGT	21	20	42.9	50.0	56.0	57.8	0.0	0.0	3.219469	49538	50728	1304-1321	1.000000	57.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GAGCTAAAACTCCCGAAATGAA	AGAAGGTCTACGGTTCGAGT	22	20	40.9	50.0	56.7	57.8	0.0	0.0	3.488514	49538	50728	1304-1321	1.000000	55.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AGCTAAAACTCCCGAAATGA	AGAAGGTCTACGGTTCGAGT	20	20	40.0	50.0	54.7	57.8	0.0	0.0	3.532674	49538	50728	1303-1320	1.000000	54.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GAGCTAAAACTCCCGAAATGA	GAAGGTCTACGGTTCGAGTC	21	20	42.9	55.0	56.0	57.5	0.0	0.0	3.563241	49538	50728	1303-1320	1.000000	54.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	AGCTAAAACTCCCGAAATGAA	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	55.5	57.8	0.0	0.0	3.694343	49538	50728	1303-1320	1.000000	53.6
accD_p1	accD	1	AGGCTACCAAATGGAGTCCT	CTAAAGGCACCAAAACAGCG	20	20	50.0	50.0	58.0	57.9	11.3	0.0	0.077889	56486	57949	2800-2826	1.000000	81.0
accD_p2	accD	2	GCCGTAGCTAATCGAGTAGC	CTAAAGGCACCAAAACAGCG	20	20	55.0	50.0	58.0	57.9	31.7	0.0	0.084055	56486	57949	2891-2917	1.000000	80.9
accD_p3	accD	3	GGCTACCAAATGGAGTCCTG	CTAAAGGCACCAAAACAGCG	20	20	55.0	50.0	58.0	57.9	11.3	0.0	0.094600	56486	57949	2799-2825	1.000000	80.9
accD_p4	accD	4	AGGCTACCAAATGGAGTCCT	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	58.0	57.9	11.3	0.0	0.130949	56486	57949	2837-2863	1.000000	80.6
accD_p5	accD	5	AGGCTACCAAATGGAGTCCT	GGGACGGCTTTTATGTGACT	20	20	50.0	50.0	58.0	57.9	11.3	0.0	0.136837	56486	57949	2250-2262	1.000000	80.5
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62418	63200	903-921	1.000000	79.7
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62418	63200	893-911	1.000000	79.7
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62418	63200	941-959	1.000000	77.9
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62418	63200	940-958	1.000000	77.9
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62418	63200	931-949	1.000000	77.9
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	65747	65910	243-244	1.000000	81.5
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	65747	65910	244-245	1.000000	81.5
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	AGAAGATACGAATCGCGCTC	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	5.6	0.0	0.139081	65747	65910	289-290	1.000000	81.1
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GAAGATACGAATCGCGCTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	5.6	0.0	0.139081	65747	65910	288-289	1.000000	81.1
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GAAGATACGAATCGCGCTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	5.6	0.0	0.140425	65747	65910	289-290	1.000000	81.1
petD_p1	petD	1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	75756	76934	1313-1319	1.000000	77.6
petD_p2	petD	2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.367124	75756	76934	1312-1318	1.000000	77.6
petD_p3	petD	3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	75756	76934	1316-1322	1.000000	77.3
petD_p4	petD	4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.402129	75756	76934	1315-1321	1.000000	77.3
petD_p5	petD	5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	20	20	45.0	50.0	57.7	58.4	0.0	0.0	0.692034	75756	76934	1316-1322	1.000000	75.0
ycf1_p1	ycf1	1	GGGGACCGGCTCAAATAAAT	TGTTTATGTGGAATGTTCTCCCA	20	23	50.0	39.1	57.9	58.0	0.0	0.0	3.075589	110017	110136	214-214	1.000000	62.1
ycf1_p2	ycf1	2	GGGGACCGGCTCAAATAAAT	GTTTATGTGGAATGTTCTCCCA	20	22	50.0	40.9	57.9	56.3	0.0	0.0	3.736764	110017	110136	213-213	1.000000	56.9
ycf1_p3	ycf1	3	GGGGACCGGCTCAAATAAAT	TGTTTATGTGGAATGTTCTCCC	20	22	50.0	40.9	57.9	56.3	0.0	0.0	3.736764	110017	110136	214-214	1.000000	56.8
ycf1_p4	ycf1	4	ACCGGCTCAAATAAATCCCA	TGTTTATGTGGAATGTTCTCCCA	20	23	45.0	39.1	57.2	58.0	0.0	0.0	3.826975	110017	110136	210-210	1.000000	56.2
ycf1_p5	ycf1	5	ATCTATGCGCACTCAAAGGC	TGTTTATGTGGAATGTTCTCCCA	20	23	50.0	39.1	58.7	58.0	14.9	0.0	3.704914	110017	110136	249-249	1.000000	56.1
psaC-ndhD_p1	psaC-ndhD	1	ACAGAGGATTGTGTCGGTTG	GGAACCCGCAAAGATTGGTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.340853	119750	119879	295-301	1.000000	80.2
psaC-ndhD_p2	psaC-ndhD	2	CAGAGGATTGTGTCGGTTGT	GGAACCCGCAAAGATTGGTA	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.340853	119750	119879	294-300	1.000000	80.2
psaC-ndhD_p3	psaC-ndhD	3	AAGAGATGTGAATCCGCCTG	GGAACCCGCAAAGATTGGTA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.493376	119750	119879	274-280	1.000000	79.2
psaC-ndhD_p4	psaC-ndhD	4	GCCCAACGGATTTCTTGAGT	GGAACCCGCAAAGATTGGTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.636292	119750	119879	255-261	1.000000	78.3
psaC-ndhD_p5	psaC-ndhD	5	GAACAGAGGATTGTGTCGGT	GGAACCCGCAAAGATTGGTA	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.628387	119750	119879	297-303	1.000000	77.9
ndhD-ccsA_p1	ndhD-ccsA	1	GGTATTGGCATGTATCCCGA	TGGATATGCTATTTTGGGGTCA	20	22	50.0	40.9	57.1	57.2	0.0	0.0	3.656402	121383	121633	413-431	1.000000	54.6
ndhD-ccsA_p2	ndhD-ccsA	2	AGGTATTGGCATGTATCCCG	TGGATATGCTATTTTGGGGTCA	20	22	50.0	40.9	57.1	57.2	0.0	0.0	3.664305	121383	121633	414-432	1.000000	54.5
ndhD-ccsA_p3	ndhD-ccsA	3	TGGCATGTATCCCGATTTTGT	TGGATATGCTATTTTGGGGTCA	21	22	42.9	40.9	58.0	57.2	0.0	0.0	3.816706	121383	121633	408-426	1.000000	53.3
ndhD-ccsA_p4	ndhD-ccsA	4	GGTATTGGCATGTATCCCGA	TGGATATGCTATTTTGGGGTCAA	20	23	50.0	39.1	57.1	57.9	0.0	0.0	3.987330	121383	121633	413-431	1.000000	51.9
ndhD-ccsA_p5	ndhD-ccsA	5	GGTATTGGCATGTATCCCGA	TTGGATATGCTATTTTGGGGTCA	20	23	50.0	39.1	57.1	57.9	0.0	0.0	3.987330	121383	121633	414-432	1.000000	51.9