Hotspot

rps16

LSC · 5355-6679 · 1325 bp

Back to Xanthosoma

Pi 0.0267

MarkerSeek score 64.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1325
variable_sites: 34
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GGGGGCGGATCCAATTAAAA	ATCAATTCCATCCCGGTTCG	50.0	50.0	58.2	58.0	1859-1919 bp	1.0	82.3
rps16_p2	GGGGGCGGATCCAATTAAAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2582-2628 bp	1.0	81.1
rps16_p3	GGGGGCGGATCCAATTAAAA	AATCCTTCCGTCCCAGATCA	50.0	50.0	58.2	58.1	2574-2620 bp	1.0	80.8
rps16_p4	GGGGGCGGATCCAATTAAAA	ATCCTTCCGTCCCAGATCAA	50.0	50.0	58.2	58.1	2573-2619 bp	1.0	80.8
rps16_p5	GGGGGCGGATCCAATTAAAA	TCCTTCCGTCCCAGATCAAT	50.0	50.0	58.2	58.1	2572-2618 bp	1.0	80.8