Hotspot

ndhB-trnL(CAA)

IRa · 153117-154248 · 1132 bp

Back to Xanthosoma

Pi 0.011

MarkerSeek score 68.70

Primer available yes

Label ndhB-trnL(CAA)

Feature Metadata

label_name: ndhB-trnL(CAA)
feature_type: igs
region: IRa
length_bp: 1132
variable_sites: 12
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1192-1274 bp	1.0	83.0
ndhB-trnL(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1190-1272 bp	1.0	83.0
ndhB-trnL(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	1187-1269 bp	1.0	81.6
ndhB-trnL(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	1191-1273 bp	1.0	80.9
ndhB-trnL(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	1189-1271 bp	1.0	80.8