Hotspot

ndhG-ndhI

SSC · 127164-127532 · 369 bp

Back to Xanthosoma

Pi 0.0797
MarkerSeek score 76.30
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
369
variable_sites
29
pi
0.0797
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TGTATTGGCCCAGTTAAATCCA ACCTCTTTATTCAAGGACAGT 40.9 38.1 57.7 54.2 423-443 bp 1.0 46.3
ndhG-ndhI_p2 TGTATTGGCCCAGTTAAATCCA CCTCTTTATTCAAGGACAGTTACC 40.9 41.7 57.7 57.0 422-442 bp 1.0 46.3
ndhG-ndhI_p3 TGTATTGGCCCAGTTAAATCCA AACCTCTTTATTCAAGGACAGT 40.9 36.4 57.7 55.0 424-444 bp 1.0 46.3
ndhG-ndhI_p4 TGTATTGGCCCAGTTAAATCCA ACCTCTTTATTCAAGGACAGTT 40.9 36.4 57.7 55.0 423-443 bp 1.0 46.3
ndhG-ndhI_p5 TGTATTGGCCCAGTTAAATCCA AACCTCTTTATTCAAGGACAGTT 40.9 34.8 57.7 55.8 424-444 bp 1.0 46.3