Hotspot

ycf1-ndhF

SSC · 117278-117697 · 420 bp

Back to Xanthosoma

Pi 0.0746
MarkerSeek score 82.60
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
420
variable_sites
25
pi
0.0746
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 TCTGGAACAATAAGAAGAACT TTGCGGGTGAACTAATTAAA 33.3 35.0 52.1 53.1 614-658 bp 1.0 47.1
ycf1-ndhF_p2 TCTGGAACAATAAGAAGAACT TTTGCGGGTGAACTAATTAA 33.3 35.0 52.1 53.1 615-659 bp 1.0 47.1
ycf1-ndhF_p3 TCTGGAACAATAAGAAGAACTT TTGCGGGTGAACTAATTAAA 31.8 35.0 53.0 53.1 614-658 bp 1.0 47.1
ycf1-ndhF_p4 TCTGGAACAATAAGAAGAACTT TTTGCGGGTGAACTAATTAA 31.8 35.0 53.0 53.1 615-659 bp 1.0 47.1
ycf1-ndhF_p5 TTCTGGAACAATAAGAAGAACT TTGCGGGTGAACTAATTAAA 31.8 35.0 53.0 53.1 615-659 bp 1.0 47.1