Hotspot

trnL(CAA)-ndhB

IRb · 101004-102135 · 1132 bp

Back to Xanthosoma

Pi 0.011

MarkerSeek score 68.70

Primer available yes

Label trnL(CAA)-ndhB

Feature Metadata

label_name: trnL(CAA)-ndhB
feature_type: igs
region: IRb
length_bp: 1132
variable_sites: 12
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(CAA)-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1192-1274 bp	1.0	83.0
trnL(CAA)-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1190-1272 bp	1.0	83.0
trnL(CAA)-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	1187-1269 bp	1.0	81.6
trnL(CAA)-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	1191-1273 bp	1.0	80.9
trnL(CAA)-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	1189-1271 bp	1.0	80.8