Hotspot

rps4-trnT(UGU)

LSC · 49508-50334 · 827 bp

Back to Xanthosoma

Pi 0.0301

MarkerSeek score 62.80

Primer available yes

Label rps4-trnT(UGU)

Feature Metadata

label_name: rps4-trnT(UGU)
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 14
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.56
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT(UGU)_p1	CCGAGCGCAATTGGTTTTTA	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.7	57.9	648-1005 bp	1.0	81.6
rps4-trnT(UGU)_p2	ACTTCCGGATCTGGGTCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	623-980 bp	1.0	81.6
rps4-trnT(UGU)_p3	CCGAGCGCAATTGGTTTTTA	GATGGTCATCGGTTCGACTC	45.0	55.0	57.7	58.2	617-974 bp	1.0	81.4
rps4-trnT(UGU)_p4	ACTTCCGGATCTGGGTCTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.3	58.2	592-949 bp	1.0	81.4
rps4-trnT(UGU)_p5	CCGAGCGCAATTGGTTTTTA	CCGCTTAGCTCAGAGGTTAG	45.0	55.0	57.7	57.5	656-1013 bp	1.0	78.6