Hotspot

rpoB-trnC(GCA)

LSC · 28669-30115 · 1447 bp

Back to Xanthosoma

Pi 0.0107
MarkerSeek score 68.00
Primer available yes
Label rpoB-trnC(GCA)

Feature Metadata

label_name
rpoB-trnC(GCA)
feature_type
igs
region
LSC
length_bp
1447
variable_sites
15
pi
0.0107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC(GCA)_p1 CCCTCATTTTCATCCCGGAG TGGGGTTTAGATCAGCAGGA 55.0 50.0 58.0 58.0 1565-1588 bp 1.0 82.7
rpoB-trnC(GCA)_p2 CTCATTTTCATCCCGGAGCA TGGGGTTTAGATCAGCAGGA 50.0 50.0 58.0 58.0 1563-1586 bp 1.0 82.5
rpoB-trnC(GCA)_p3 CCCTCATTTTCATCCCGGAG GCGACACCCAGATTTGAACT 55.0 50.0 58.0 58.5 1516-1539 bp 1.0 79.2
rpoB-trnC(GCA)_p4 CTCATTTTCATCCCGGAGCA GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1514-1537 bp 1.0 79.1
rpoB-trnC(GCA)_p5 CCCTCATTTTCATCCCGGAG TAAAGGATTTGCAGTCCCCC 55.0 50.0 58.0 57.5 1491-1514 bp 1.0 78.9