Hotspot

trnK(UUU)-rps16

LSC · 4564-5354 · 791 bp

Back to Xanthosoma

Pi 0.0181
MarkerSeek score 71.10
Primer available yes
Label trnK(UUU)-rps16

Feature Metadata

label_name
trnK(UUU)-rps16
feature_type
igs
region
LSC
length_bp
791
variable_sites
14
pi
0.0181
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 879-884 bp 1.0 81.2
trnK(UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT AGCTCAACCCACAAGAACTG 50.0 50.0 58.2 58.0 878-883 bp 1.0 81.2
trnK(UUU)-rps16_p3 CCGCACTTAAAAGCCGAGTA AGCTCAACCCACAAGAACTG 50.0 50.0 58.3 58.0 888-893 bp 1.0 80.2
trnK(UUU)-rps16_p4 AAAGCCGAGTACTCTACCGT GAGCTCAACCCACAAGAACT 50.0 50.0 58.2 57.7 880-885 bp 1.0 79.3
trnK(UUU)-rps16_p5 AAGCCGAGTACTCTACCGTT GAGCTCAACCCACAAGAACT 50.0 50.0 58.2 57.7 879-884 bp 1.0 79.3