Hotspot

tRNA-GUG-psbA

LSC · 68-562 · 495 bp

Back to Xanthosoma

Pi 0.0251
MarkerSeek score 73.70
Primer available yes
Label tRNA-GUG-psbA

Feature Metadata

label_name
tRNA-GUG-psbA
feature_type
igs
region
LSC
length_bp
495
variable_sites
11
pi
0.0251
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-GUG-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 616-619 bp 1.0 83.0
tRNA-GUG-psbA_p2 GATCCACTTGGCTACATCCG ATCAACCGTGCTAACCTTGG 55.0 50.0 58.1 58.2 618-621 bp 1.0 83.0
tRNA-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 630-633 bp 1.0 82.9
tRNA-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 629-632 bp 1.0 82.9
tRNA-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 631-634 bp 1.0 82.9