Hotspot

ycf1

SSC · 108700-113850 · 5151 bp

Back to Xanthium

Pi 0.0235

MarkerSeek score 58.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5151
variable_sites: 119
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAGAATACCGTCACCCATT	CAGCCGTTTCTGTGCAATTT	50.0	45.0	58.0	57.9	753-831 bp	1.0	87.5
ycf1_p2	CTAGCACTGGGTAGACCTCA	CAGCCGTTTCTGTGCAATTT	55.0	45.0	57.9	57.9	1204-1282 bp	1.0	85.1
ycf1_p3	GCCGAGAAGAAGGTATCAGC	CAGCCGTTTCTGTGCAATTT	55.0	45.0	58.1	57.9	1288-1366 bp	1.0	85.0
ycf1_p4	AGAAGAAGGAGCCGAGAAGA	CAGCCGTTTCTGTGCAATTT	50.0	45.0	57.8	57.9	1298-1376 bp	1.0	84.3
ycf1_p5	AAGGAGCCGAGAAGAAGGTA	CAGCCGTTTCTGTGCAATTT	50.0	45.0	57.8	57.9	1293-1371 bp	1.0	84.2