Hotspot

rpl2

IRa · 150761-152247 · 1487 bp

Back to Xanthium

Pi 0.00202

MarkerSeek score 45.70

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1487
variable_sites: 3
pi: 0.00202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TCGGGGAAAGGCTAGAAGAA	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1707-1712 bp	1.0	73.6
rpl2_p2	TTCGGGGAAAGGCTAGAAGA	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1708-1713 bp	1.0	73.6
rpl2_p3	GGTGTCAAGGTAATCGCGAT	GAACGTGTCACAGCTTCCTC	50.0	55.0	58.1	58.9	1742-1747 bp	1.0	73.5
rpl2_p4	CTATGACCCGCTAAGCATCC	GAACGTGTCACAGCTTCCTC	55.0	55.0	57.9	58.9	2124-2129 bp	1.0	73.2
rpl2_p5	GCATTGGGTCGAACTCTTCT	GAACGTGTCACAGCTTCCTC	50.0	55.0	57.9	58.9	1764-1769 bp	1.0	73.2