Hotspot

rpl32-ndhF

SSC · 124263-125063 · 801 bp

Back to Xanthium

Pi 0.0164

MarkerSeek score 61.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 801
variable_sites: 13
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CTTTTAACGCCGCCCCATAT	CTGGAAGTGGAAGAAAAGGGA	50.0	47.6	58.7	57.5	935-944 bp	1.0	64.4
rpl32-ndhF_p2	TTTTAACGCCGCCCCATATC	CTGGAAGTGGAAGAAAAGGGA	50.0	47.6	58.7	57.5	934-943 bp	1.0	64.4
rpl32-ndhF_p3	GCCCCATATCCTTTCCCTTT	CTGGAAGTGGAAGAAAAGGGA	50.0	47.6	57.2	57.5	924-933 bp	1.0	63.7
rpl32-ndhF_p4	CTTTTAACGCCGCCCCATAT	AGAAAAGGGATTATCCACGCA	50.0	42.9	58.7	57.4	924-933 bp	1.0	63.3
rpl32-ndhF_p5	TTTTAACGCCGCCCCATATC	AGAAAAGGGATTATCCACGCA	50.0	42.9	58.7	57.4	923-932 bp	1.0	63.3