Hotspot

rps19-rpl2

IRb · 84287-84364 · 78 bp

Back to Xanthium

Pi 0.127

MarkerSeek score 76.80

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 78
variable_sites: 9
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	ATAATGGTAGATGCCCGGGA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	312-319 bp	1.0	83.8
rps19-rpl2_p2	ATAATGGTAGATGCCCGGGA	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	349-356 bp	1.0	83.5
rps19-rpl2_p3	ATAATGGTAGATGCCCGGGA	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.0	58.0	529-536 bp	1.0	82.4
rps19-rpl2_p4	ATAATGGTAGATGCCCGGGA	GATGATGGAACGGGAAGACC	50.0	55.0	58.0	58.0	849-854 bp	1.0	81.1
rps19-rpl2_p5	TATCGCAAAACTACGCCCTC	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.1	58.0	1064-1071 bp	1.0	80.8