Hotspot

ycf1

IRa · 133270-136908 · 3639 bp

Back to Wurfbainia

Pi 0.00319

MarkerSeek score 35.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 3639
variable_sites: 27
pi: 0.00319
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGTTTTTCGGGTAAAAATGT	TTTCTGAACCAAAATTACAACT	31.8	27.3	55.0	52.4	185 bp	1.0	43.0
ycf1_p2	AGTGTTTTTCGGGTAAAAATGT	ATTTCTGAACCAAAATTACAACT	31.8	26.1	55.0	53.1	186 bp	1.0	43.0
ycf1_p3	GTGTTTTTCGGGTAAAAATGT	TTTCTGAACCAAAATTACAACT	33.3	27.3	53.5	52.4	184 bp	1.0	43.0
ycf1_p4	AGTGTTTTTCGGGTAAAAATGTAGA	TTTCTGAACCAAAATTACAACT	32.0	27.3	57.4	52.4	185 bp	1.0	43.0
ycf1_p5	ATCAATAGTGTTTTTCGGGT	TTTCTGAACCAAAATTACAACT	35.0	27.3	52.8	52.4	191 bp	1.0	42.9