Hotspot

ndhA

SSC · 128025-130152 · 2128 bp

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Pi 0.00455

MarkerSeek score 61.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2128
variable_sites: 21
pi: 0.00455
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCAGTCACCATCGGAAACAT	GTCCACCCGGCTTCATAAAT	50.0	50.0	57.9	57.9	2361-2380 bp	1.0	79.7
ndhA_p2	CATGAACCCAGTCACCATCG	GTCCACCCGGCTTCATAAAT	55.0	50.0	58.6	57.9	2368-2387 bp	1.0	75.6
ndhA_p3	CCAGTCACCATCGGAAACAT	TCCACCCGGCTTCATAAATT	50.0	45.0	57.9	57.2	2360-2379 bp	1.0	73.6
ndhA_p4	CCCAGTCACCATCGGAAAC	GTCCACCCGGCTTCATAAAT	57.9	50.0	58.1	57.9	2362-2381 bp	1.0	71.4
ndhA_p5	CCAGTCACCATCGGAAACAT	TCCACCCGGCTTCATAAATTT	50.0	42.9	57.9	57.9	2360-2379 bp	1.0	71.3