Hotspot

atpF

LSC · 12454-13819 · 1366 bp

Back to Wurfbainia

Pi 0.00599

MarkerSeek score 38.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1366
variable_sites: 19
pi: 0.00599
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATTCCGTCGCCTACTTGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2071-2101 bp	1.0	82.0
atpF_p2	CAATTCCGTCGCCTACTTGA	AGGAATTACCGCATTCGCTT	50.0	45.0	58.0	58.0	1863-1893 bp	1.0	81.9
atpF_p3	CAATTCCGTCGCCTACTTGA	AGGCCAAGCTGTAGAAGGTA	50.0	50.0	58.0	58.0	2178-2208 bp	1.0	81.6
atpF_p4	CCGTTTCGGCTACCACAATA	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.0	58.0	2638-2668 bp	1.0	81.5
atpF_p5	CCGTTTCGGCTACCACAATA	AGGAATTACCGCATTCGCTT	50.0	45.0	58.0	58.0	2430-2460 bp	1.0	81.4