Hotspot

psaC-ndhE

SSC · 125587-126258 · 672 bp

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Pi 0.00493

MarkerSeek score 57.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 672
variable_sites: 7
pi: 0.00493
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	CCGAAGCAGCTATTGGACTT	55.0	50.0	58.4	58.0	800-861 bp	1.0	81.1
psaC-ndhE_p2	CGTCCCACGGTATCATTTCC	TGTTTCGTCGATTCACCGAA	55.0	45.0	58.4	57.9	775-836 bp	1.0	80.2
psaC-ndhE_p3	CGTCCCACGGTATCATTTCC	TTCGTCGATTCACCGAAACA	55.0	45.0	58.4	57.9	772-833 bp	1.0	80.2
psaC-ndhE_p4	CGTCCCACGGTATCATTTCC	TTGTTTCGTCGATTCACCGA	55.0	45.0	58.4	57.9	776-837 bp	1.0	80.2
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	CCGAAGCAGCTATTGGACTT	50.0	50.0	58.6	58.0	767-828 bp	1.0	80.0