Hotspot

ccsA-ndhD

SSC · 123424-123709 · 286 bp

Back to Wurfbainia

Pi 0.0136

MarkerSeek score 51.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 286
variable_sites: 9
pi: 0.0136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGCACTTGTGGCTTCTATGG	GGGACTGGTATTTATCCCGA	50.0	50.0	57.9	56.1	482 bp	1.0	65.3
ccsA-ndhD_p2	ATTCAGCACTTGTGGCTTCT	GGGACTGGTATTTATCCCGA	45.0	50.0	57.7	56.1	486 bp	1.0	64.0
ccsA-ndhD_p3	ACAAATTCAGCACTTGTGGC	GGGACTGGTATTTATCCCGA	45.0	50.0	57.5	56.1	490 bp	1.0	62.1
ccsA-ndhD_p4	AGCACTTGTGGCTTCTATGG	GGGACTGGTATTTATCCCGAT	50.0	47.6	57.9	56.6	482 bp	1.0	61.7
ccsA-ndhD_p5	ATTCAGCACTTGTGGCTTCT	GGGACTGGTATTTATCCCGAT	45.0	47.6	57.7	56.6	486 bp	1.0	60.4