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rpl32-trnL-TAG

SSC · 121383-122271 · 889 bp

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Pi 0.0103

MarkerSeek score 44.70

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 889
variable_sites: 22
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	CAAACAAATAATAAAGTCGTGGA	AGCAGCGTGTCTACCAATTT	30.4	45.0	53.7	57.8	948-951 bp	1.0	43.9
rpl32-trnL-TAG_p2	CAAACAAATAATAAAGTCGTGGA	CCTAAGAGCAGCGTGTCTAC	30.4	55.0	53.7	57.8	954-957 bp	1.0	43.9
rpl32-trnL-TAG_p3	CAAACAAATAATAAAGTCGTGGA	CTAAGAGCAGCGTGTCTACC	30.4	55.0	53.7	57.8	953-956 bp	1.0	43.9
rpl32-trnL-TAG_p4	CAAACAAATAATAAAGTCGTGGA	GAGCAGCGTGTCTACCAATT	30.4	50.0	53.7	58.3	949-952 bp	1.0	43.9
rpl32-trnL-TAG_p5	CAAACAAATAATAAAGTCGTGGA	TCCTAAGAGCAGCGTGTCTA	30.4	50.0	53.7	57.9	955-958 bp	1.0	43.8