Hotspot

psaJ-rpl33

LSC · 71224-71881 · 658 bp

Back to Wurfbainia

Pi 0.00467

MarkerSeek score 55.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 7
pi: 0.00467
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCCTTGTCATTCCCAT	TTTCGGGCACAATTGGTACA	50.0	45.0	57.9	58.0	731-748 bp	1.0	81.9
psaJ-rpl33_p2	ATCGTTTATTCCCGGATGCC	TTTCGGGCACAATTGGTACA	50.0	45.0	58.1	58.0	744-761 bp	1.0	81.6
psaJ-rpl33_p3	GGATGCCTTGTCATTCCCAT	TCGGGCACAATTGGTACATT	50.0	45.0	57.9	57.8	729-746 bp	1.0	80.3
psaJ-rpl33_p4	GGATGCCTTGTCATTCCCAT	TTCGGGCACAATTGGTACAT	50.0	45.0	57.9	57.8	730-747 bp	1.0	80.3
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	TTTCGGGCACAATTGGTACA	50.0	45.0	57.7	58.0	781-798 bp	1.0	80.0