Hotspot

cemA-petA

LSC · 65817-66069 · 253 bp

Back to Wurfbainia

Pi 0.0126

MarkerSeek score 53.70

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 6
pi: 0.0126
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCTTCGCTTGT	GGATCGAGTCATCCCTTCCT	50.0	55.0	57.7	58.3	352 bp	1.0	76.8
cemA-petA_p2	CGTGTATCTCCTTCGCTTGT	CGCAAAAACGGGATATGCAT	50.0	45.0	57.7	57.5	421 bp	1.0	75.0
cemA-petA_p3	TCGTGTATCTCCTTCGCTTG	GGATCGAGTCATCCCTTCCT	50.0	55.0	57.4	58.3	353 bp	1.0	74.6
cemA-petA_p4	CGTGTATCTCCTTCGCTTGT	TGGATCGAGTCATCCCTTCC	50.0	55.0	57.7	58.6	353 bp	1.0	74.4
cemA-petA_p5	TCGTGTATCTCCTTCGCTTG	CGCAAAAACGGGATATGCAT	50.0	45.0	57.4	57.5	422 bp	1.0	72.7