Hotspot

ndhC-trnM-CAT

LSC · 53847-56410 · 2564 bp

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Pi 0.00851
MarkerSeek score 55.40
Primer available yes
Label ndhC-trnM-CAT

Feature Metadata

label_name
ndhC-trnM-CAT
feature_type
igs
region
LSC
length_bp
2564
variable_sites
42
pi
0.00851
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAT_p1 CCTAACCGGGGCTAAAACTC TTGAACCAATGACTACCGCC 55.0 50.0 58.0 58.2 2635-2756 bp 1.0 82.3
ndhC-trnM-CAT_p2 CTAACCGGGGCTAAAACTCC TTGAACCAATGACTACCGCC 55.0 50.0 58.0 58.2 2634-2755 bp 1.0 82.3
ndhC-trnM-CAT_p3 GGGGCTAAAACTCCGGAAAT TTGAACCAATGACTACCGCC 50.0 50.0 57.9 58.2 2628-2749 bp 1.0 81.5
ndhC-trnM-CAT_p4 CTAACCGGGGCTAAAACTCC ACCGCCGTATGAAAGCAATA 55.0 45.0 58.0 57.7 2620-2741 bp 1.0 81.2
ndhC-trnM-CAT_p5 CTAACCGGGGCTAAAACTCC TACCGCCGTATGAAAGCAAT 55.0 45.0 58.0 57.7 2621-2742 bp 1.0 81.2