Hotspot

psaA-ycf3

LSC · 44713-45365 · 653 bp

Back to Wurfbainia

Pi 0.00249

MarkerSeek score 50.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 3
pi: 0.00249
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	AAGATCACGAGGCGTTTTGA	50.0	45.0	58.5	57.8	771-782 bp	1.0	76.4
psaA-ycf3_p2	TGTTCTTGAGAAATGGCCGG	AGATCACGAGGCGTTTTGAA	50.0	45.0	58.5	57.8	770-781 bp	1.0	76.4
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	TGAAGATCACGAGGCGTTTT	50.0	45.0	58.5	57.8	773-784 bp	1.0	76.4
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	774-785 bp	1.0	76.4
psaA-ycf3_p5	TTTAACTTCTGGTTCCGGCG	AGATCACGAGGCGTTTTGAA	50.0	45.0	58.5	57.8	698-709 bp	1.0	76.4