Hotspot

trnS-TGA-psbZ

LSC · 38501-38867 · 367 bp

Back to Wurfbainia

Pi 0.00545

MarkerSeek score 38.00

Primer available yes

Label trnS-TGA-psbZ

Feature Metadata

label_name: trnS-TGA-psbZ
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 5
pi: 0.00545
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-TGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ACCAACTGTCAGAAGAAGCA	55.0	45.0	58.0	57.0	488-499 bp	1.0	72.6
trnS-TGA-psbZ_p2	GAGCTATCAACCACTCAGCC	ACCAACTGTCAGAAGAAGCA	55.0	45.0	58.1	57.0	495-506 bp	1.0	72.3
trnS-TGA-psbZ_p3	GGAGCTATCAACCACTCAGC	ACCAACTGTCAGAAGAAGCA	55.0	45.0	58.1	57.0	496-507 bp	1.0	72.3
trnS-TGA-psbZ_p4	CAAGACCGGAGCTATCAACC	ACCAACTGTCAGAAGAAGCA	55.0	45.0	58.1	57.0	503-514 bp	1.0	72.3
trnS-TGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GACCAACTGTCAGAAGAAGCA	55.0	47.6	58.0	58.2	489-500 bp	1.0	71.4