Hotspot

trnT-GGT-psbD

LSC · 34512-35832 · 1321 bp

Back to Wurfbainia

Pi 0.00859

MarkerSeek score 48.60

Primer available yes

Label trnT-GGT-psbD

Feature Metadata

label_name: trnT-GGT-psbD
feature_type: igs
region: LSC
length_bp: 1321
variable_sites: 25
pi: 0.00859
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGT-psbD_p1	GTGGTAGAGTAACGCCATGG	TCAAATAGATCATTTTCTTCTTTGGT	55.0	26.9	58.1	55.7	1422-1429 bp	1.0	43.0
trnT-GGT-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCAAATAGATCATTTTCTTCTTTGGT	50.0	26.9	58.1	55.7	1425-1432 bp	1.0	43.0
trnT-GGT-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCAAATAGATCATTTTCTTCTTTGGT	50.0	26.9	58.3	55.7	1399-1406 bp	1.0	43.0
trnT-GGT-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAATAGATCATTTTCTTCTTTGGT	55.0	28.0	58.1	54.3	1421-1428 bp	1.0	43.0
trnT-GGT-psbD_p5	GGCCCTTTTAACTCAGTGGT	TCAAATAGATCATTTTCTTCTTTGGT	50.0	26.9	57.7	55.7	1437-1444 bp	1.0	42.9