Hotspot

trnE-TTC-trnT-GGT

LSC · 33529-34439 · 911 bp

Back to Wurfbainia

Pi 0.00964

MarkerSeek score 52.50

Primer available yes

Label trnE-TTC-trnT-GGT

Feature Metadata

label_name: trnE-TTC-trnT-GGT
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 17
pi: 0.00964
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-trnT-GGT_p1	TCCTTTCGTAGTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	955-1050 bp	1.0	84.2
trnE-TTC-trnT-GGT_p2	TCCTTTCGTAGTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	934-1029 bp	1.0	83.7
trnE-TTC-trnT-GGT_p3	TCCTTTCGTAGTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	931-1026 bp	1.0	83.6
trnE-TTC-trnT-GGT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	919-1014 bp	1.0	83.3
trnE-TTC-trnT-GGT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	898-993 bp	1.0	83.2