Hotspot

rps16-trnQ-TTG

LSC · 5977-7326 · 1350 bp

Back to Wurfbainia

Pi 0.00531

MarkerSeek score 50.90

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1350
variable_sites: 16
pi: 0.00531
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1424-1452 bp	1.0	80.0
rps16-trnQ-TTG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1435-1463 bp	1.0	79.5
rps16-trnQ-TTG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1428-1456 bp	1.0	78.9
rps16-trnQ-TTG_p4	AAGTCGCACGTTGCTTTCTA	AGGATAAAAATGGGGCGTGG	45.0	50.0	58.1	58.2	1482-1510 bp	1.0	78.7
rps16-trnQ-TTG_p5	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1439-1467 bp	1.0	78.4