Hotspot

rps4-trnL-UAA

LSC · 44882-45537 · 656 bp

Back to Woodwardia

Pi 0.0487

MarkerSeek score 82.30

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 46
pi: 0.0487
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCACTTTCAAACGAGGTCCTC	ATTGCCCGATAAATGGTCCC	47.6	50.0	57.9	58.0	790-812 bp	1.0	77.7
rps4-trnL-UAA_p2	TCACTTTCAAACGAGGTCCTC	CTGCCACTTGATACGTCTCC	47.6	55.0	57.9	58.1	749-771 bp	1.0	77.5
rps4-trnL-UAA_p3	TCACTTTCAAACGAGGTCCTC	CTCAATCTAGCTGAGTCCGC	47.6	55.0	57.9	57.9	720-742 bp	1.0	77.3
rps4-trnL-UAA_p4	TCACTTTCAAACGAGGTCCTC	GCTCAATCTAGCTGAGTCCG	47.6	55.0	57.9	57.9	721-743 bp	1.0	77.3
rps4-trnL-UAA_p5	TCACTTTCAAACGAGGTCCTC	CAATCTAGCTGAGTCCGCTG	47.6	55.0	57.9	58.1	718-740 bp	1.0	77.2